Simultaneous Detection , Genotyping and Quantification of 1 Human Papillomaviruses by Multicolor Real - Time PCR and 2 Melting Curve Analysis 3

Simultaneous Detection, Genotyping and Quantification of 1 Human Papillomaviruses by Multicolor Real-Time PCR and 2 Melting Curve Analysis 3 Yiqun Liao†, Yulin Zhou†, Qiwei Guo, Xiaoting Xie, Ena Luo, Jian Li * and Qingge Li * 4 School of Pharmaceutical Sciences, Xiamen University, Xiamen 361005, Fujian, China; 5 Molecular Diagnostics Laboratory, Department of Genetics, Prenatal Diagnosis Center of Xiamen, 6 Maternal and Child Health Hospital of Xiamen, Xiamen 361003, Fujian, China; 7 Engineering Research Center of Molecular Diagnostics, Ministry of Education, Department of 8 Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China. 9 †These authors contributed equally to this work. 10 * Correspondence authors: 11 J. Li, Molecular Diagnostics Laboratory, Department of Genetics, Prenatal Diagnosis Center of 12 Xiamen, Maternal and Child Health Hospital of Xiamen, Xiamen 361003, Fujian, China. Fax 13 86-592-2662032; e-mail: [email protected]. 14 Q. Li, Engineering Research Center of Molecular Diagnostics, Ministry of Education, Department of 15 Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China. 16 Fax 86-592-2187363; [email protected]. 17 18 Running title: Real-Time PCR Genotyping for 30 HPV types 19